Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage

Abstract

Tissue culture is a method of propagation plants by isolating cell, tissues, protoplasts or plant organs than planting them in a sterile and controlled environment. This method is able to produce superior plants and propagation of plants are difficult to cultivate conventionallyin large quantities with a relatively fast time. Acclimatization is an important final stage in the success of tissue culture method. Acclimatization is carried out for adaptation of the plantlet environment from in vitro to in vivo conditions. This is caused in the previous stage the plantlets are heterotrophic, while on acclimatization stage it will be au totrophic. This study aims to provide an information about the factors that affect the success of acclimatization plantlets. This is a review article was conducted using the literature study method by collecting information from journals, books or internet than analyzed in descriptive qualitative to prove scientifically. On this stage, many plantlets fail to grow and even die. for increase growth and reduce plantlets mortality need to be made overcome these problems. This can be overcome by applying acclima tization supporting factors. The result that factors affecting of the success plantlet growth on acclimatization stage includ ing: the condition of the plantlets, light intensity, temperature, relative humidity, types of planting media, nutrition and fertil izing and controlling of pest and microorganisms.